The story starts with Takiya Genji, the son of a Yakuza boss, entering Suzuran All-Boys High School as a transferred senior with the goal of conquering it. During the Freshers' orientation lecture, Yakuza arrive at the school seeking Tamao Serizawa to avenge an injury.

Crows Zero is a spin-off based on Hiroshi Takahashi's manga Crows, but the storyline happens before it, so it can be seen as a prequel to the original. The series was adapted into a Japanese live-action film that was released on October 27, 2007.