Amu Hinamori is a shy girl who struggles to make friends in part due to the rumors spread around school about her "cool and spicy" personality. Wishing that she could show everyone her "would-be" self, Amu wakes up to the presence of three colorful and ornately decorated eggs in her bed. Each of these "Hearts Eggs" houses a "Guardian Character"—manifestations of someone's would-be self and their dreams. With the aid of Ran, Miki, and Su, Amu now finds herself able to express who she truly is. However, things do not always pan out in her favor. The sudden appearance of Amu's Guardian Characters prompts the Seiyo Elementary Guardians, a student council-like group with each member possessing their own Guardian Character, to take notice of her. As someone who has multiple Characters, Amu is roped into being the Guardians' "Joker," tasked with helping them find X Eggs—corrupted versions of one's Hearts Egg. As she becomes entangled in the Guardians' quest, Amu begins to uncover more about these mysterious Characters, the X Eggs, and the organization that seems to be at the center of it all. [Written by MAL Rewrite]

Shugo Chara! was released in 16 compilation DVDs by Pony Canyon, each containing three to four episodes, from February 20, 2008 to May 20, 2009. The series has been dubbed in French and Italian by Studio La Dame Blanche and LOGOS srl respectively.